Biomedical research
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Automated High-Throughput PCR Clean-up with NucleoSpin® 96 PCR Clean-up and Andrew+
MACHEREY-NAGEL
This advanced method offers an end-to-end solution developed around an advanced application, a specialized kit, or an analytical system. It delivers complete functional, ready-to-use protocols that are qualitatively and quantitatively assessed for consistency, executability, and repeatability, usually without needing to make any changes. It operates per batch size and is scalable to accommodate changing requirements.
PCR clean-up is a time-consuming, error-prone step that limits throughput in cloning and sequencing workflows, especially when many samples must be processed at once. Automating the silica membrane-based NucleoSpin® 96 PCR Clean-up kit with the Andrew+ robot and Extraction+ vacuum module addresses this by handling 8–96 samples in parallel without manual pipetting or vacuum steps. The kit recovers 75–95 % of DNA fragments from PCRs and enzymatic reactions, delivering clean DNA ready for cloning and sequencing.
Automated High-Throughput RNA Purification with NucleoSpin® 96 RNA and Andrew+
MACHEREY-NAGEL
This advanced method offers an end-to-end solution developed around an advanced application, a specialized kit, or an analytical system. It delivers complete functional, ready-to-use protocols that are qualitatively and quantitatively assessed for consistency, executability, and repeatability, usually without needing to make any changes. It operates per batch size and is scalable to accommodate changing requirements.
High-throughput RNA purification is a common bottleneck in transcriptomics and molecular biology workflows. The silica membrane-based NucleoSpin® 96 RNA kit, run on the Andrew+ robot with the Extraction+ vacuum module, automates purification of 8–96 samples in parallel with on-column DNase treatment, eliminating repetitive pipetting and manual vacuum steps. It delivers high-integrity RNA (RIN up to 10) with typical yields of 10–40 µg from cells and tissue, reducing errors and streamlining downstream analysis.
Automated Transfection-Grade Plasmid DNA Purification with NucleoSpin® 96 Plasmid TG Plus and Andrew+
MACHEREY-NAGEL
This advanced method offers an end-to-end solution developed around an advanced application, a specialized kit, or an analytical system. It delivers complete functional, ready-to-use protocols that are qualitatively and quantitatively assessed for consistency, executability, and repeatability, usually without needing to make any changes. It operates per batch size and is scalable to accommodate changing requirements.
High endotoxin can compromise cell viability in transfection work, and manual purification limits throughput. Automating the NucleoSpin® 96 Plasmid Transfection-grade Plus kit with Andrew+ and Extraction+ delivers high yields with very low endotoxin (≤ 10 EU/µg, 3 washes; ≤ 1 EU/µg, 4 washes) for fast, parallel transfection-grade DNA isolation.
High-Throughput Automated Plasmid DNA Purification with NucleoSpin® 96 Plasmid and Andrew+
MACHEREY-NAGEL
This advanced method offers an end-to-end solution developed around an advanced application, a specialized kit, or an analytical system. It delivers complete functional, ready-to-use protocols that are qualitatively and quantitatively assessed for consistency, executability, and repeatability, usually without needing to make any changes. It operates per batch size and is scalable to accommodate changing requirements.
Plasmid purification often slows cloning and sequencing pipelines. Pairing the NucleoSpin® 96 Plasmid kit with Andrew+ and Extraction+ processes up to 96 samples simultaneously with excellent reproducibility, no manual pipetting or vacuum handling. It delivers cloning-grade plasmid DNA (> 50 EU/µg), yielding 4–30 µg per 1–5 mL culture.
This basic method provides the core methodology for translating a workflow into OneLab-executable script(s) as an attempt to fully or semi-automate a specific procedure. It demonstrates the benefits of automation and highlights OneLab capabilities and best practices to promote solution adoption, helping transition from manual to a more automated approach. It can be used alone or serves as a building block for a more complex workflow and is easily adaptable to users' requirements.
To analyze amino acids in biological samples by LC-MS, Waters provides a fast reproducible solution that uses pre-column derivatization built on the AccQ•Tag reaction chemistry, with the ability to quantitate over 40 amino acids in less than 10 minutes.
Waters Kairos Amino Acid Kit – High Throughput (500+) enables precise and accurate preparation of over 40 amino acids using the Andrew+ Pipetting Robot.
