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Standard Curve Generation
This basic method provides the core methodology for translating a workflow into OneLab-executable script(s) as an attempt to fully or semi-automate a specific procedure. It demonstrates the benefits of automation and highlights OneLab capabilities and best practices to promote solution adoption, helping transition from manual to a more automated approach. It can be used alone or serves as a building block for a more complex workflow and is easily adaptable to users' requirements.

Published by
Waters Corporation
Version 4, 16 Sep 2022 at 6:44 AM
LC-MS analytical method development requires flexible and reproducible sample preparation for accurate and robust analyte quantification.
This method provides example protocols of automated sample preparation for standard curve generation, using the Andrew+ Pipetting Robot with cloud-native OneLab software, ensuring downstream accurate and reproducible LC-MS quantification of different analytes.
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Please note that this is a “Tutorial Method”
This basic method provides the core methodology for translating a workflow into OneLab-executable script(s) as an attempt to fully or semi-automate a specific procedure. It demonstrates the benefits of automation and highlights OneLab capabilities and best practices to promote solution adoption, helping transition from manual to a more automated approach. It can be used alone or serves as a building block for a more complex workflow and is easily adaptable to users' requirements.
Overview
Protocols
5

Standard Curve Preparation - Nitrosamine Impurities
This method is for the creation of a calibration curve for nitrosamine impurities by the Andrew+ Pipetting Robot for quantification by LC-MS analysis.

Final Dilution - Carnitine by LC-MS/MS
This method is for the final dilution of samples using the Andrew+ Pipetting Robot for quantification of carnitine by LC-MS/MS analysis.

Final Dilution - Cysteine & Methionine by LC-FLR
This method is for the final dilution of samples using the Andrew+ Pipetting Robot for quantification of cysteine and methionine by LC-FLR analysis.

Standard Curve Preparation - Galactose by IC-ED
This method is for the creation of a calibration curve for galactose using the Andrew+ Pipetting Robot for quantification of galactose by IC-ED analysis.

Standard Curve Preparation - Vitamin A by LC-FLR
This method is for the creation of a calibration curve for vitamin A (retinol) using the Andrew+ Pipetting Robot for quantification of vitamin A by LC-FLR analysis.
Contact info

Published by
Waters Corporation
From the everyday consumer to scientists in the laboratory, we all rely on accurate information to make critical decisions. Waters Corporation is the world's leading specialty measurement company focused on improving human health and well-being through the application of high-value analytical technologies and industry leading scientific expertise.
This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 