Simple Serial Dilution Preparation
This example method provides a freely adjustable framework for measuring the adaptability of the OneLab environment to workflows from different application fields. It helps to understand various nuances of the code-free, universal protocol designer and provides general indications as to the feasibility of a project. Scripts generally require fine adjustment to correct for variables and support specific labware implementation.
Published by
Waters Corporation
Version 1, 18 Nov 2022 at 4:41 PM
Workflows like ELISA assays, protein content determination and quantitative LC-MS analysis rely on serial dilutions to prepare calibration curves and quality control samples.
This series of methods demonstrates simple 2- and 5-fold serial dilutions in 96-well plates in a row or column-wise fashion. The same script can be executed in a fully automated manner using Andrew+ or guided using Pipette+.
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Please note that this is a “Get Started Method”
This example method provides a freely adjustable framework for measuring the adaptability of the OneLab environment to workflows from different application fields. It helps to understand various nuances of the code-free, universal protocol designer and provides general indications as to the feasibility of a project. Scripts generally require fine adjustment to correct for variables and support specific labware implementation.
Protocols
4
2-Fold Serial Dilution by Column
This protocol sets out the procedure to perform a 2-fold serial dilution in triplicate using the columns of a 96-well plate.
5-Fold Serial Dilution by Column
This protocol sets out the procedure to perform a 5-fold serial dilution in triplicate using the columns of a 96-well plate.
2-Fold Serial Dilution by Row
This protocol sets out the procedure to perform a 2-fold serial dilution in triplicate using the rows of a 96-well plate.
5-Fold Serial Dilution by Row
This protocol sets out the procedure to perform a 5-fold serial dilution in triplicate using the rows of a 96-well plate.
Contact info
Published by
Waters Corporation
From the everyday consumer to scientists in the laboratory, we all rely on accurate information to make critical decisions. Waters Corporation is the world's leading specialty measurement company focused on improving human health and well-being through the application of high-value analytical technologies and industry leading scientific expertise.
This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 