QSC Fluorescent Antibody Titration
This example method provides a freely adjustable framework for measuring the adaptability of the OneLab environment to workflows from different application fields. It helps to understand various nuances of the code-free, universal protocol designer and provides general indications as to the feasibility of a project. Scripts generally require fine adjustment to correct for variables and support specific labware implementation.
Published by
Andrew Alliance
Version 1, 16 Sep 2022 at 6:44 AM
The expression level of specific receptors serves as a biomarker for pathological conditions. The quantitation of their number on target cells is very informative from a diagnostic and therapeutic perspective.
Consistent and repeatable sample preparation and staining of calibration beads and cells are ensured through accurate pipetting using OneLab, thereby minimizing variation in ABC assignments.
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Please note that this is a “Get Started Method”
This example method provides a freely adjustable framework for measuring the adaptability of the OneLab environment to workflows from different application fields. It helps to understand various nuances of the code-free, universal protocol designer and provides general indications as to the feasibility of a project. Scripts generally require fine adjustment to correct for variables and support specific labware implementation.
Protocols
2
QSC Microsphere Antibody Titration
This protocol will guide you in the preparation of a serial dilution of the fluorophore-conjugated mAb, the staining of level 3 QSC beads and finally the preparation of samples for flow cytometry analysis.
Analysis of Stained QSC Microspheres to Create a Standard Curve
This protocol enables you to stain the QSC standards (level 1 to 4, excluding blank) using the optimal antibody concentration determined in protocol 1 and to prepare labeled QSC beads for analysis in order to establish a standard curve.
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Published by
Andrew Alliance
Reproducibility and traceability for your laboratory, starting from serial dilution design. Connected devices for your lab, allowing you to run daily experiments with effiiciency. affordable automation. Error-free pipetting. Save your time. save your health.
This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 