Plasmid Purification and Clean-up
This basic method provides the core methodology for translating a workflow into OneLab-executable script(s) as an attempt to fully or semi-automate a specific procedure. It demonstrates the benefits of automation and highlights OneLab capabilities and best practices to promote solution adoption, helping transition from manual to a more automated approach. It can be used alone or serves as a building block for a more complex workflow and is easily adaptable to users' requirements.
Published by
MACHEREY-NAGEL
Version 1, 19 Aug 2026 at 4:49 PM
Plasmid purification, PCR Clean-up and DNA extraction from gels are the most commonly used applications in molecular biology labs. Both applications are also important steps in the cloning workflow.
The MACHEREY-NAGEL NucleoSpin Plasmid and NucleoSpin Gel and PCR Clean-up kit in combination with Pipette+ is the easiest way to purify isolate plasmid DNA and DNA fragments from agarose gels or PCR reactions.
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Please note that this is a “Tutorial Method”
This basic method provides the core methodology for translating a workflow into OneLab-executable script(s) as an attempt to fully or semi-automate a specific procedure. It demonstrates the benefits of automation and highlights OneLab capabilities and best practices to promote solution adoption, helping transition from manual to a more automated approach. It can be used alone or serves as a building block for a more complex workflow and is easily adaptable to users' requirements.
Protocols
3
Plasmid DNA Isolation, 6 Samples - Pipette+
Small-scale preparation of highly pure plasmid DNA from E. coli (Miniprep) using MACHEREY-NAGEL NucleoSpin® Plasmid, Mini kit (REF: 740588.10/.50/.250).
DNA PCR Clean-up, 6 Samples - Pipette+
DNA purification from PCR mixtures using MACHEREY-NAGEL NucleoSpin® Gel and PCR Clean-up, Mini kit (REF: 740609.10/.50/.250).
DNA Extraction from Agarose Gel, 6 Samples - Pipette+
DNA Extraction from agarose gel samples using MACHEREY-NAGEL NucleoSpin® Gel and PCR Clean-up, Mini kit (REF: 740609.10/.50/.250).
Contact info
Published by
MACHEREY-NAGEL
MACHEREY-NAGEL was founded in 1911 in Düren as a specialist manufacturer of filter papers. It has evolved into an innovative international company for chemical and biomolecular analysis. It has subsidiaries in Germany, Switzerland, France and the U.S. It has worldwide sales in 150 countries, and has more than 25,000 products in application areas that include water filtration, rapid test, analysis, chromatography, and bioanalysis tools, including DNA and RNA purification products.
This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 