PeptideWorks Tryptic Protein Digestion Sample Prep
This advanced method offers an end-to-end solution developed around an advanced application, a specialized kit, or an analytical system. It delivers complete functional, ready-to-use protocols that are qualitatively and quantitatively assessed for consistency, executability, and repeatability, usually without needing to make any changes. It operates per batch size and is scalable to accommodate changing requirements.
Published by
Waters Corporation
Version 3, 17 Jul 2023 at 8:47 AM
Reproducible peptide maps are valuable for the routine analysis of biotherapeutic proteins in R&D, QC, and manufacturing environments.
Here, we present an automated comprehensive sample prep protocol using the PeptideWorks™ sample preparation kit and the Andrew+ Pipetting Robot. The automated method provides a reproducible protein digest and is suitable for commercial and research environments.
Get in touch with an expert about running this protocol on Andrew+.
Please note that this is a “Click & Execute Method”
This advanced method offers an end-to-end solution developed around an advanced application, a specialized kit, or an analytical system. It delivers complete functional, ready-to-use protocols that are qualitatively and quantitatively assessed for consistency, executability, and repeatability, usually without needing to make any changes. It operates per batch size and is scalable to accommodate changing requirements.
Protocols
9
PeptideWorks Protocol A, 4 Samples, with Conc. Norm.
Protocol for denaturation, reduction, alkylation, and desalt of 4 protein samples with preparation for concentration normalization.
PeptideWorks Protocol A, 4 Samples, without Conc. Norm.
Protocol for denaturation, reduction, alkylation, and desalt of 4 protein samples without preparation for concentration normalization.
PeptideWorks Protocol A, 24 Samples, with Conc. Norm.
Protocol for denaturation, reduction, alkylation, and desalt of 24 protein samples with preparation for concentration normalization.
PeptideWorks Protocol A, 24 Samples, without Conc. Norm.
Protocol for denaturation, reduction, alkylation, and desalt of 24 protein samples without preparation for concentration normalization.
PeptideWorks Protocol B, 4 Samples, with Conc. Norm.
Concentration normalization and trypsin digestion of 4 denatured and desalted protein samples.
PeptideWorks Protocol B, 4 Samples, without Conc. Norm.
Trypsin digestion of 4 denatured and desalted protein samples.
PeptideWorks Protocol B, 24 Samples, with Conc. Norm.
Concentration normalization and trypsin digestion of 24 denatured and desalted protein samples.
PeptideWorks Protocol B, 24 Samples, without Conc. Norm.
Trypsin digestion of 24 denatured and desalted protein samples.
PeptideWorks, Reaction Plate Preparation
Dynamic protocol for the preparation of the PeptideWorks reaction plate. Transfers protein samples from a 96-well PCR plate to a Waters 96-well 700 uL sample collection plate. Can be adapted for 1 to 24 samples.
Contact info
Published by
Waters Corporation
From the everyday consumer to scientists in the laboratory, we all rely on accurate information to make critical decisions. Waters Corporation is the world's leading specialty measurement company focused on improving human health and well-being through the application of high-value analytical technologies and industry leading scientific expertise.
This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 