OligoWorks™ Sample Pretreatment and SPE Extraction
This advanced method offers an end-to-end solution developed around an advanced application, a specialized kit, or an analytical system. It delivers complete functional, ready-to-use protocols that are qualitatively and quantitatively assessed for consistency, executability, and repeatability, usually without needing to make any changes. It operates per batch size and is scalable to accommodate changing requirements.
Published by
Waters Corporation
Version 1, 12 Oct 2023 at 6:59 PM
Sample preparation for therapeutic oligonucleotide (ONT) bioanalysis can be challenging, requiring extensive method development. Some workflows, like liquid-liquid extraction (LLE) can be difficult to automate, while others may use detergent-based reagents, requiring extensive washing and sample evaporation/reconstitution to make it LC-MS compatible.
OligoWorks Kits are simple, standardized, flexible and automation-friendly sample preparation kits designed to enable accurate and robust LC-MS based bioanalytical quantitation across a diversity of oligonucleotides.
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Please note that this is a “Click & Execute Method”
This advanced method offers an end-to-end solution developed around an advanced application, a specialized kit, or an analytical system. It delivers complete functional, ready-to-use protocols that are qualitatively and quantitatively assessed for consistency, executability, and repeatability, usually without needing to make any changes. It operates per batch size and is scalable to accommodate changing requirements.
Protocols
3
OligoWorks pretreatment – Rapizyme Proteinase K Digestion method
Dynamic protocol for detergent-free, enzyme-based digestion pretreatment of biofluid samples for Oligonucleotide bioanalysis prior to SPE extraction using the OligoWorks Rapizyme Proteinase K Digestion module.
OligoWorks WAX SPE Microplate method
Dynamic protocol for extracting therapeutic Oligonucleotides from biofluids using OligoWorks WAX SPE Microplate using a standardized protocol provided in the Care & Use manual, optimized for 100 µL sample volume.
OligoWorks full workflow (pretreatment and SPE)
This protocol combined both Rapizyme Proteinase K Digestion sample pretreatment and SPE using OligoWorks WAX SPE Microplate.
Contact info
Published by
Waters Corporation
From the everyday consumer to scientists in the laboratory, we all rely on accurate information to make critical decisions. Waters Corporation is the world's leading specialty measurement company focused on improving human health and well-being through the application of high-value analytical technologies and industry leading scientific expertise.
This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 