O-glycopeptide Profile Analysis
This advanced method offers an end-to-end solution developed around an advanced application, a specialized kit, or an analytical system. It delivers complete functional, ready-to-use protocols that are qualitatively and quantitatively assessed for consistency, executability, and repeatability, usually without needing to make any changes. It operates per batch size and is scalable to accommodate changing requirements.
Published by
Genovis
Version 1, 24 May 2023 at 12:32 PM
Developing a robust process for manufacturing biopharmaceuticals poses many challenges. It requires high-throughput methods with fast sample prep, reproducible analytics, and easy data interpretation.
Here we present an automated sample prep workflow to compare the O-glycopeptide profiles of heavily O-glycosylated protein therapeutics using the O-protease OpeRATOR for subsequent LC-MS analysis.
Get in touch with an expert about running this protocol on Andrew+.
Please note that this is a “Click & Execute Method”
This advanced method offers an end-to-end solution developed around an advanced application, a specialized kit, or an analytical system. It delivers complete functional, ready-to-use protocols that are qualitatively and quantitatively assessed for consistency, executability, and repeatability, usually without needing to make any changes. It operates per batch size and is scalable to accommodate changing requirements.
Protocols
3
O-glycopeptide Generation, Fc-fusion Proteins, 2 Samples, 3 Replicates
Protocol 1/2: Digestion of the target protein using the enzymes OpeRATOR, SialEXO, and FabRICATOR in a one-pot reaction into glycopeptides with an N-terminal core 1 O-glycan. FabRICATOR is added to cleave off the Fc region.
O-glycopeptide Generation, Other Proteins, 2 Samples, 3 Replicates
Protocol 1/2: Digestion of the target protein using the enzymes OpeRATOR and SialEXO in a one-pot reaction into glycopeptides with an N-terminal core 1 O-glycan.
O-glycopeptide Reduction and Alkylation, 2 Samples, 3 Replicates
Protocol 2/2: Reduction of peptide disulfide bonds with DTT, alkylation with iodoacetamide, and stopping of reaction with formic acid.
Contact info
Published by
Genovis
Genovis is a Swedish biotech company, and we are dedicated to discovering new enzymes and providing them as tools for applications in the biopharma industry. Our SmartEnzymes portfolio consists of enzymes for Antibody Digestion, Antibody Deglycosylation, Antibody Labeling, Glycan Profiling, Affinity Purification and Proteomics.
This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 