KAPA DNA Library Quantification
This example method provides a freely adjustable framework for measuring the adaptability of the OneLab environment to workflows from different application fields. It helps to understand various nuances of the code-free, universal protocol designer and provides general indications as to the feasibility of a project. Scripts generally require fine adjustment to correct for variables and support specific labware implementation.
Published by
Andrew Alliance
Version 1, 16 Sep 2022 at 6:44 AM
Accurate quantification of Illumina NGS libraries is crucial to obtaining ideal cluster density and equal sample representation in the library pool.
The reliability and reproducibility of qPCR measurements depend on accurate pipetting during sample dilutions and qPCR reaction setup. OneLab helps in minimizing user-related variations in liquid handling by providing full guidance and traceability.
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Please note that this is a “Get Started Method”
This example method provides a freely adjustable framework for measuring the adaptability of the OneLab environment to workflows from different application fields. It helps to understand various nuances of the code-free, universal protocol designer and provides general indications as to the feasibility of a project. Scripts generally require fine adjustment to correct for variables and support specific labware implementation.
Protocols
1
KAPA Library Quantification using KAPA SYBR FAST qPCR Master Mix
This quick start protocol describes the dilution of two DNA libraries and the internal control followed by the qPCR reaction setup using KAPA SYBR FAST qPCR Master Mix with appropriate primers and without a passive reference dye (ROX).
Contact info
Published by
Andrew Alliance
Reproducibility and traceability for your laboratory, starting from serial dilution design. Connected devices for your lab, allowing you to run daily experiments with effiiciency. affordable automation. Error-free pipetting. Save your time. save your health.
This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 