Intracellular Flow Cytometry
This example method provides a freely adjustable framework for measuring the adaptability of the OneLab environment to workflows from different application fields. It helps to understand various nuances of the code-free, universal protocol designer and provides general indications as to the feasibility of a project. Scripts generally require fine adjustment to correct for variables and support specific labware implementation.
Published by
Andrew Alliance
Version 2, 16 Sep 2022 at 6:44 AM
Standardizing cell preparation for flow cytometry analysis is an effective way to ensure optimal permeabilization and staining of cells, thereby resulting in consistent fluorescence intensity and event detection rate.
In line with this objective, OneLab provides valuable resources to guide sample preparation and liquid handling, ensuring the delivery of reproducible results.
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Please note that this is a “Get Started Method”
This example method provides a freely adjustable framework for measuring the adaptability of the OneLab environment to workflows from different application fields. It helps to understand various nuances of the code-free, universal protocol designer and provides general indications as to the feasibility of a project. Scripts generally require fine adjustment to correct for variables and support specific labware implementation.
Protocols
1
Intracellular Staining Flow Cytometry Protocol
This quick start protocol is intended for direct immunostaining of intracellular molecular targets in fixed cells for flow cytometry analysis. It has been developed and optimized by R&D Systems Flow Cytometry Laboratory.
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Published by
Andrew Alliance
Reproducibility and traceability for your laboratory, starting from serial dilution design. Connected devices for your lab, allowing you to run daily experiments with effiiciency. affordable automation. Error-free pipetting. Save your time. save your health.
This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 