Indirect Sandwich ELISA
This example method provides a freely adjustable framework for measuring the adaptability of the OneLab environment to workflows from different application fields. It helps to understand various nuances of the code-free, universal protocol designer and provides general indications as to the feasibility of a project. Scripts generally require fine adjustment to correct for variables and support specific labware implementation.
Published by
Andrew Alliance
Version 3, 24 Aug 2023 at 7:46 PM
Sandwich ELISA is the most robust and sensitive type of ELISA technique. ELISA protocols traditionally have a complex workflow that requires optimization to obtain accurate results with low analytical error.
To maximize accuracy and reproducibility, OneLab can guide you through your optimized protocol to ensure consistent pipetting throughout the assay and between sample replicates.
Get in touch with an expert about running this protocol on Andrew+.
Please note that this is a “Get Started Method”
This example method provides a freely adjustable framework for measuring the adaptability of the OneLab environment to workflows from different application fields. It helps to understand various nuances of the code-free, universal protocol designer and provides general indications as to the feasibility of a project. Scripts generally require fine adjustment to correct for variables and support specific labware implementation.
Protocols
4
ELISA – Protein Standard Preparation, Pipette+
This protocol enables you to make eight-point, two-fold serial dilution of the recombinant protein standard (stock solution 100 μg/mL) at final concentrations spanning 0 (blank) to 1000 pg/mL using the standard diluent.
ELISA – Plate Coating, Blocking, and Sample-Standard Addition
In this protocol, wells are coated with capture antibody and blocked using BSA solution before the addition of samples and standards in duplicate. After incubation, wells are thoroughly washed to retain only specific binding events.
ELISA – Incubation with Detection Antibody and Enzyme Conjugate
In this protocol, samples and standards are incubated first with biotinylated detection antibody and then with enzyme conjugate at the appropriate concentration. Proper washing ensures that the signal is due solely to the specific binding events.
ELISA – Substrate Addition and Colorimetric Detection
This protocol describes the procedure for signal detection. It begins with adding the substrate solution and stopping the reaction once the desired color intensity is reached. The absorbance is measured and a standard curve is finally established.
Contact info
Published by
Andrew Alliance
Reproducibility and traceability for your laboratory, starting from serial dilution design. Connected devices for your lab, allowing you to run daily experiments with effiiciency. affordable automation. Error-free pipetting. Save your time. save your health.
This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 