Guided NucleoBond Xtra Maxiprep
This basic method provides the core methodology for translating a workflow into OneLab-executable script(s) as an attempt to fully or semi-automate a specific procedure. It demonstrates the benefits of automation and highlights OneLab capabilities and best practices to promote solution adoption, helping transition from manual to a more automated approach. It can be used alone or serves as a building block for a more complex workflow and is easily adaptable to users' requirements.
Published by
MACHEREY-NAGEL
Version 2, 19 Aug 2026 at 4:49 PM
With our long-standing experience in chromatography and nucleic acid purification, MACHEREY-NAGEL is an ideal choice for high-quality plasmid preps.
Using NucleoBond Xtra Maxi, yields of up to 1000 μg of ultrapure plasmid DNA can be obtained based on reliable and well-established anion exchange chromatography.
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Please note that this is a “Tutorial Method”
This basic method provides the core methodology for translating a workflow into OneLab-executable script(s) as an attempt to fully or semi-automate a specific procedure. It demonstrates the benefits of automation and highlights OneLab capabilities and best practices to promote solution adoption, helping transition from manual to a more automated approach. It can be used alone or serves as a building block for a more complex workflow and is easily adaptable to users' requirements.
Protocols
3
Guided NucleoBond Xtra Maxi, 4 Samples
Isolation of transfection-grade plasmid DNA (4 samples) using Pipette+ and MACHEREY-NAGEL's NucleoBond Xtra Maxi kit without desalting step.
Guided NucleoBond Xtra Maxi 4 Samples + Desalting Beads
Isolation of transfection-grade plasmid DNA (4 samples) using Pipette+ and MACHEREY-NAGEL's NucleoBond Xtra Maxi kit including a magnetic bead-based desalting step.
Desalting of 4 Plasmid Maxi Eluates Using Magnetic Beads
Desalting of anion exchange Maxi eluates using Pipette+ and MACHEREY-NAGEL's NucleoMag Desalting Beads.
Contact info
Published by
MACHEREY-NAGEL
MACHEREY-NAGEL was founded in 1911 in Düren as a specialist manufacturer of filter papers. It has evolved into an innovative international company for chemical and biomolecular analysis. It has subsidiaries in Germany, Switzerland, France and the U.S. It has worldwide sales in 150 countries, and has more than 25,000 products in application areas that include water filtration, rapid test, analysis, chromatography, and bioanalysis tools, including DNA and RNA purification products.
This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 