GlycoWorks® N-Glycan Automated Sample Prep
This advanced method offers an end-to-end solution developed around an advanced application, a specialized kit, or an analytical system. It delivers complete functional, ready-to-use protocols that are qualitatively and quantitatively assessed for consistency, executability, and repeatability, usually without needing to make any changes. It operates per batch size and is scalable to accommodate changing requirements.
Published by
Waters Corporation
Version 6, 2 Apr 2024 at 8:41 AM
Monitoring N-Glycosylation is increasingly applied in the development of biopharmaceuticals and the study of diseased cells. Glycan preparation is laborious, and analytical methods lack sensitivity.
GlycoWorks RapiFluor-MS accelerates N-glycan preparation while improving detection sensitivity. Repeatability and traceability are assured when paired with Waters Lab automation solutions.
Get in touch with an expert about running this protocol on Andrew+.
Please note that this is a “Click & Execute Method”
This advanced method offers an end-to-end solution developed around an advanced application, a specialized kit, or an analytical system. It delivers complete functional, ready-to-use protocols that are qualitatively and quantitatively assessed for consistency, executability, and repeatability, usually without needing to make any changes. It operates per batch size and is scalable to accommodate changing requirements.
Protocols
8
GlycoWorks Sample Prep, 8 samples
Step 1b + 2 • GlycoWorks sample preparation protocol for processing 8 samples from the 24-sample kit using Andrew+.
GlycoWorks Sample Prep, 24 samples
Step 1b + 2 • GlycoWorks sample preparation protocol for processing 24 samples from the 96-sample kit using Andrew+.
GlycoWorks Sample Prep, 48 samples
Step 1b + 2 • GlycoWorks sample preparation protocol for processing 48 samples from the 96-sample high throughput automation kit using Andrew+.
GlycoWorks Sample Prep - Step 1a + 1b (w. Diafiltration), 48 Samples
Step 1a + 1b • GlycoWorks sample preparation protocol for releasing and labeling glycans from 48 samples using Andrew+. Includes a diafiltration step for sample buffer exchange or concentration.
GlycoWorks Sample Prep - Step 2 (Cleanup), 48 Samples
Step 2 • GlycoWorks sample preparation cleanup protocol 48 samples using Andrew+.
GlycoWorks Reagent Prep, 8 samples of 24 sample Kit (Pipette+)
Guided protocol for manually reconstituting the GlycoWorks reagents of a 24-sample kit using Pipette+ to process 8 samples.
GlycoWorks Reagent Prep, 24 samples of 96 sample kit (Pipette+)
Guided protocol for manually reconstituting the GlycoWorks reagents of a 96-sample kit using Pipette+ to process 24 samples.
GlycoWorks Reagent Prep, 48 samples of 96 sample kit (Pipette+)
Guided protocol for manually reconstituting the GlycoWorks reagents of a 96-sample high throughput automation kit using Pipette+ to process 48 samples.
Contact info
Published by
Waters Corporation
From the everyday consumer to scientists in the laboratory, we all rely on accurate information to make critical decisions. Waters Corporation is the world's leading specialty measurement company focused on improving human health and well-being through the application of high-value analytical technologies and industry leading scientific expertise.
This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 