DNA Sequencing Library Preparation
This example method provides a freely adjustable framework for measuring the adaptability of the OneLab environment to workflows from different application fields. It helps to understand various nuances of the code-free, universal protocol designer and provides general indications as to the feasibility of a project. Scripts generally require fine adjustment to correct for variables and support specific labware implementation.
Published by
Andrew Alliance
Version 1, 16 Sep 2022 at 6:44 AM
Library preparation aims to build a collection of DNA fragments for next-generation sequencing (NGS). A high-quality DNA library guarantees uniform and consistent genome coverage, thus delivering comprehensive and reliable sequencing data.
OneLab helps you streamline and trace critical steps in your library preparation process to achieve the best possible DNA quality and purity.
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Please note that this is a “Get Started Method”
This example method provides a freely adjustable framework for measuring the adaptability of the OneLab environment to workflows from different application fields. It helps to understand various nuances of the code-free, universal protocol designer and provides general indications as to the feasibility of a project. Scripts generally require fine adjustment to correct for variables and support specific labware implementation.
Protocols
2
DNA Tagmentation, Cleanup and Amplification - 24 Samples
This protocol, based on Illumina's Nextera Flex library prep, uses on-bead reaction to fragment and tag DNA simultaneously. Adapter-ligated DNA fragments are then washed before DNA amplification. Only early steps of PCR preparation are included.
Library Purification - 24 Samples
This protocol, based on Illumina's Nextera Flex library prep, is dedicated for the purification of amplified DNA libraries prior to sequencing.
Contact info
Published by
Andrew Alliance
Reproducibility and traceability for your laboratory, starting from serial dilution design. Connected devices for your lab, allowing you to run daily experiments with effiiciency. affordable automation. Error-free pipetting. Save your time. save your health.
This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 