CRISPR/Cas9-mediated Gene Knockout
This example method provides a freely adjustable framework for measuring the adaptability of the OneLab environment to workflows from different application fields. It helps to understand various nuances of the code-free, universal protocol designer and provides general indications as to the feasibility of a project. Scripts generally require fine adjustment to correct for variables and support specific labware implementation.
Published by
Andrew Alliance
Version 2, 16 Sep 2022 at 6:44 AM
Gene Knockout is a technique that is fundamental to the investigation of gene function, which has been made easier and faster by the revolutionary breakthrough of CRISPR technology.
OneLab provides clear step-by-step guidance on correct execution of the clonal isolation process to ensure optimal input, as well as identification of wells with single colonies. Additionally, it records each step in these procedures, enabling full traceability.
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Please note that this is a “Get Started Method”
This example method provides a freely adjustable framework for measuring the adaptability of the OneLab environment to workflows from different application fields. It helps to understand various nuances of the code-free, universal protocol designer and provides general indications as to the feasibility of a project. Scripts generally require fine adjustment to correct for variables and support specific labware implementation.
Protocols
1
Isolation of Single Cell-derived Knocked-out Clones
This protocol, based on GeneCopoeia's protocol for CRISPR-mediated gene knock-out without a donor, enables you to grow colonies from individual cells using the serial dilution method. The goal is to isolate a clonal cell line with a stable mutation.
Contact info
Published by
Andrew Alliance
Reproducibility and traceability for your laboratory, starting from serial dilution design. Connected devices for your lab, allowing you to run daily experiments with effiiciency. affordable automation. Error-free pipetting. Save your time. save your health.
This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 