Automated ProA Resin Based Antibody Purification
This advanced method offers an end-to-end solution developed around an advanced application, a specialized kit, or an analytical system. It delivers complete functional, ready-to-use protocols that are qualitatively and quantitatively assessed for consistency, executability, and repeatability, usually without needing to make any changes. It operates per batch size and is scalable to accommodate changing requirements.
Published by
Waters Corporation
Version 1, 19 Nov 2025 at 7:54 AM
An automated Protein A affinity purification method for up to 96 culture media samples in a well plate format is presented. The automation uses Cytiva antibody purification resin1 on Andrew+ liquid handling robot.
The purification consumes 120 µL HCCF to generate 100 µL of antibody in neutral pH buffer solution. Dynamic processing of 96 samples demonstrated excellent recovery and reproducibility.
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Please note that this is a “Click & Execute Method”
This advanced method offers an end-to-end solution developed around an advanced application, a specialized kit, or an analytical system. It delivers complete functional, ready-to-use protocols that are qualitatively and quantitatively assessed for consistency, executability, and repeatability, usually without needing to make any changes. It operates per batch size and is scalable to accommodate changing requirements.
Protocols
2
ProA-resin purification 96-well plate
200uL media containing mAb (120uL consumed)
60uL resin, 96 wells
Labware: 8019 Pall 350uL 0.2um
ProA-resin purification 96-well plate with Shaker+
120uL mAb, 60uL resin, 96 wells
Based on 7/23 run, 200uL media
Contact info
Published by
Waters Corporation
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This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 