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Automated Plate & Cartridge RP-SPE
This basic method provides the core methodology for translating a workflow into OneLab-executable script(s) as an attempt to fully or semi-automate a specific procedure. It demonstrates the benefits of automation and highlights OneLab capabilities and best practices to promote solution adoption, helping transition from manual to a more automated approach. It can be used alone or serves as a building block for a more complex workflow and is easily adaptable to users' requirements.

Published by
Waters Corporation
Version 1, 24 Aug 2023 at 7:46 PM
This work demonstrates the capabilities of the Andrew+ Pipetting Robot configured with the Extraction+ Connected Device for fully automated SPE sample preparation using cartridge and plate formats.
Described Protocols use a simple 3-step SPE procedure (load, wash, and elution) in combination with Waters reversed-phase Oasis HLB cartridges (1, 3, and 6 cc size) and 96-well macro and μElution plates.
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Please note that this is a “Tutorial Method”
This basic method provides the core methodology for translating a workflow into OneLab-executable script(s) as an attempt to fully or semi-automate a specific procedure. It demonstrates the benefits of automation and highlights OneLab capabilities and best practices to promote solution adoption, helping transition from manual to a more automated approach. It can be used alone or serves as a building block for a more complex workflow and is easily adaptable to users' requirements.
Overview
Protocols
5

Automated Oasis HLB 1 cc Cartridge Reversed-Phase SPE
This protocol demonstrates how the Andrew+ Pipetting Robot configured with Extraction+ autonomously prepared and extracted samples using Oasis HLB 1 cc Cartridge (Waters, p/n 186001879) and following a simple 3-step SPE protocol (load, wash & elute).

Automated Oasis HLB 3 cc Cartridge Reversed-Phase SPE
This protocol demonstrates how the Andrew+ Pipetting Robot configured with Extraction+ autonomously prepared and extracted samples using Oasis HLB 3 cc Cartridge (Waters, p/n 186001880) and following a simple 3-step SPE protocol (load, wash & elute).

Automated Oasis HLB 6 cc Cartridge Reversed-Phase SPE
This protocol demonstrates how the Andrew+ Pipetting Robot configured with Extraction+ autonomously prepared and extracted samples using Oasis HLB 6 cc Cartridge (Waters, p/n 186003379) and following a simple 3-step SPE protocol (load, wash & elute).

Automated Oasis HLB 96-well Macro Plate Reversed-Phase SPE
This protocol demonstrates how the Andrew+ Pipetting Robot configured with Extraction+ autonomously prepared and extracted samples using Oasis HLB 96x Macro Plate (Waters, p/n WAT058951) and following a 3-step SPE protocol (load, wash & elute).

Automated Oasis HLB 96-well μElution Plate Reversed-Phase SPE
This protocol demonstrates how the Andrew+ Pipetting Robot configured with Extraction+ autonomously prepared and extracted samples using Oasis HLB 96x μElution Plate (Waters, p/n 186001828BA) and following a 3-step SPE protocol (load, wash & elute).
Contact info

Published by
Waters Corporation
From the everyday consumer to scientists in the laboratory, we all rely on accurate information to make critical decisions. Waters Corporation is the world's leading specialty measurement company focused on improving human health and well-being through the application of high-value analytical technologies and industry leading scientific expertise.
This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 