Automated mAb Subunit Screening
This basic method provides the core methodology for translating a workflow into OneLab-executable script(s) as an attempt to fully or semi-automate a specific procedure. It demonstrates the benefits of automation and highlights OneLab capabilities and best practices to promote solution adoption, helping transition from manual to a more automated approach. It can be used alone or serves as a building block for a more complex workflow and is easily adaptable to users' requirements.
Published by
Waters Corporation
Version 1, 16 Sep 2022 at 6:44 AM
High-throughput analytical platforms for the characterization of proteins produced at clone selection stage have also been increasingly on demand to facilitate the development of these biopharmaceuticals.
Here we present a fully automated workflow for sample preparation and LC-MS analysis of mAbs obtained directly from unrefined samples such as spent cell culture media including host cells protein.
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Please note that this is a “Tutorial Method”
This basic method provides the core methodology for translating a workflow into OneLab-executable script(s) as an attempt to fully or semi-automate a specific procedure. It demonstrates the benefits of automation and highlights OneLab capabilities and best practices to promote solution adoption, helping transition from manual to a more automated approach. It can be used alone or serves as a building block for a more complex workflow and is easily adaptable to users' requirements.
Protocols
4
Rapid Subunit Screening, 8 Samples
Antibody clean-up and subunit digestion from conditioned cell culture media (8 samples) using Magnetic beads and IdES on the Andrew+ Pipetting Robot.
Rapid Subunit Screening, 48 Samples - Step A
Step A of antibody clean-up and subunit digestion from conditioned cell culture media (48 samples) using Magnetic beads and IdES on the Andrew+ Pipetting Robot.
Rapid Subunit Screening, 48 Samples - Step B
Step B of antibody clean-up and subunit digestion from conditioned cell culture media (48 samples) using Magnetic beads and IdES on the Andrew+ Pipetting Robot.
Rapid Subunit Screening, 48 Samples - Steps A and B
Antibody clean-up and subunit digestion from conditioned cell culture media (48 samples) using Magnetic beads and IdES on the Andrew+ Pipetting Robot.
Contact info
Published by
Waters Corporation
From the everyday consumer to scientists in the laboratory, we all rely on accurate information to make critical decisions. Waters Corporation is the world's leading specialty measurement company focused on improving human health and well-being through the application of high-value analytical technologies and industry leading scientific expertise.
This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 