Automated mAb Subunit Generation
This advanced method offers an end-to-end solution developed around an advanced application, a specialized kit, or an analytical system. It delivers complete functional, ready-to-use protocols that are qualitatively and quantitatively assessed for consistency, executability, and repeatability, usually without needing to make any changes. It operates per batch size and is scalable to accommodate changing requirements.
Published by
Genovis
Version 3, 24 May 2023 at 12:32 PM
Subunit analysis of therapeutic antibodies by LC-MS using FabRICATOR (IdeS) digestion provides a rapid approach for analyzing many important quality attributes including Fc glycosylation and oxidation.
Here, we describe protocols for automated sample preparation using the Andrew+ Pipetting Robot.
Get in touch with an expert about running this protocol on Andrew+.
Please note that this is a “Click & Execute Method”
This advanced method offers an end-to-end solution developed around an advanced application, a specialized kit, or an analytical system. It delivers complete functional, ready-to-use protocols that are qualitatively and quantitatively assessed for consistency, executability, and repeatability, usually without needing to make any changes. It operates per batch size and is scalable to accommodate changing requirements.
Protocols
2
Sample Preparation for mAb Subunit Analysis
Digestion of monoclonal antibodies with FabRICATOR with reduction of disulfide bonds in a 30 min one-pot reaction.
Sample Preparation for mAb Subunit Analysis, with deglycosylation
Digestion of monoclonal antibodies with FabRICATOR and GlycINATOR with reduction of disulfide bonds in a 30 min one-pot reaction.
Contact info
Published by
Genovis
Genovis is a Swedish biotech company, and we are dedicated to discovering new enzymes and providing them as tools for applications in the biopharma industry. Our SmartEnzymes portfolio consists of enzymes for Antibody Digestion, Antibody Deglycosylation, Antibody Labeling, Glycan Profiling, Affinity Purification and Proteomics.
This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 