Automated Bioanalytical SPE
This basic method provides the core methodology for translating a workflow into OneLab-executable script(s) as an attempt to fully or semi-automate a specific procedure. It demonstrates the benefits of automation and highlights OneLab capabilities and best practices to promote solution adoption, helping transition from manual to a more automated approach. It can be used alone or serves as a building block for a more complex workflow and is easily adaptable to users' requirements.
Published by
Waters Corporation
Version 1, 24 Aug 2023 at 7:46 PM
The following work demonstrates the capabilities of the Andrew+ Pipetting Robot in combination with the Extraction+ Connected Device for fully automated bioanalytical solid phase extraction (SPE) for a variety of pharmaceutical drugs from plasma.
Protocols for both cartridge and 96-well SPE plate formats are included. Calibration curves and QC samples are created in one protocol and extracted in a separate one.
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Please note that this is a “Tutorial Method”
This basic method provides the core methodology for translating a workflow into OneLab-executable script(s) as an attempt to fully or semi-automate a specific procedure. It demonstrates the benefits of automation and highlights OneLab capabilities and best practices to promote solution adoption, helping transition from manual to a more automated approach. It can be used alone or serves as a building block for a more complex workflow and is easily adaptable to users' requirements.
Protocols
5
Calibrators and QC Preparation for Macro Plate Extraction
This protocol demonstrates the preparation of Calibrators and QC plasma samples from 5-500 ng/mL using the Andrew+ Pipetting Robot. Prepared samples will be subsequently used in the protocol "Automated SPE using Oasis HLB 96-well Plate".
Automated SPE using Oasis HLB 96-well Plate, by Row
This protocol is intended for the automated extraction of Calibrators and QCs in plasma using Water Oasis HLB 96-well plate, 30 mg sorbent/well, 30 μm (p/n WAT058951), row-wise, with the Extraction+ Connected Device.
Automated SPE using Oasis HLB 96-well Plate, by Column
This protocol is intended for the automated extraction of Calibrators and QCs in plasma using Water Oasis HLB 96-well plate, 30 mg sorbent/well, 30 μm (p/n WAT058951), processed column-wise, with the Extraction+ Connected Device.
Calibrators and QC Preparation for 1 cc Cartridge Extraction
This protocol demonstrates the preparation of Calibrators and QC plasma samples from 5-200 ng/mL using the Andrew+ Pipetting Robot. Prepared samples will be subsequently used in the protocol "Automated SPE using Oasis HLB 1 cc Cartridges"
Automated SPE using Oasis HLB 1 cc Cartridges
This protocol is intended for the automated extraction of Calibrators and QCs in plasma using Waters Oasis HLB 1 cc Vac Cartridge, 30 mg sorbent, 30 μm (p/n WAT094225) with the Extraction+ Connected Device.
Contact info
Published by
Waters Corporation
From the everyday consumer to scientists in the laboratory, we all rely on accurate information to make critical decisions. Waters Corporation is the world's leading specialty measurement company focused on improving human health and well-being through the application of high-value analytical technologies and industry leading scientific expertise.
This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 