Analysis of mAbs in Crude Samples
This advanced method offers an end-to-end solution developed around an advanced application, a specialized kit, or an analytical system. It delivers complete functional, ready-to-use protocols that are qualitatively and quantitatively assessed for consistency, executability, and repeatability, usually without needing to make any changes. It operates per batch size and is scalable to accommodate changing requirements.
Published by
Genovis
Version 2, 24 May 2023 at 12:32 PM
Developing a robust process for manufacturing therapeutic mAbs poses many analytical challenges. This requires high-throughput methods with fast sample preparation, robust analytics, and easy data interpretation.
Here we present a fully automated workflow for sample preparation for middle-level analysis by LC-MS of mAbs directly from crude protein samples such as harvested cell culture fluid.
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Please note that this is a “Click & Execute Method”
This advanced method offers an end-to-end solution developed around an advanced application, a specialized kit, or an analytical system. It delivers complete functional, ready-to-use protocols that are qualitatively and quantitatively assessed for consistency, executability, and repeatability, usually without needing to make any changes. It operates per batch size and is scalable to accommodate changing requirements.
Protocols
8
Automated FabRICATOR MagIC digestion, 4 Samples - 37°C
Digestion of four mAb samples with FabRICATOR MagIC (37°C) to yield mAb subunits ready for analysis by LC-MS.
Automated FabRICATOR MagIC digestion, 8 Samples - 37°C
Digestion of eight mAb samples with FabRICATOR MagIC (37°C) to yield mAb subunits ready for analysis by LC-MS.
Automated FabRICATOR MagIC digestion, 4 Samples - RT
Digestion of four mAb samples with FabRICATOR MagIC (RT) to yield mAb subunits ready for analysis by LC-MS.
Automated FabRICATOR MagIC digestion, 8 Samples - RT
Digestion of eight mAb samples with FabRICATOR MagIC (RT) to yield mAb subunits ready for analysis by LC-MS.
IgG Purification + FabRICATOR MagIC digestion, 4 Samples - 37°C
Purification of antibodies (4 samples) using Protein A Mag Sepharose® Xtra (Cytiva, p/n 28967056) followed by digestion with FabRICATOR MagIC (37°C) to yield mAb subunits ready for analysis by LC-MS.
IgG Purification + FabRICATOR MagIC digestion, 8 Samples - 37°C
Purification of antibodies (8 samples) using Protein A Mag Sepharose® Xtra (Cytiva, p/n 28967056) followed by digestion with FabRICATOR MagIC (37°C) to yield mAb subunits ready for analysis by LC-MS.
IgG Purification + FabRICATOR MagIC digestion, 4 Samples - RT
Purification of antibodies (4 samples) using Protein A Mag Sepharose® Xtra (Cytiva, p/n 28967056) followed by digestion with FabRICATOR MagIC (RT) to yield mAb subunits ready for analysis by LC-MS.
IgG Purification + FabRICATOR MagIC digestion, 8 Samples - RT
Purification of antibodies (8 samples) using Protein A Mag Sepharose® Xtra (Cytiva, p/n 28967056) followed by digestion with FabRICATOR MagIC (RT) to yield mAb subunits ready for analysis by LC-MS.
Contact info
Published by
Genovis
Genovis is a Swedish biotech company, and we are dedicated to discovering new enzymes and providing them as tools for applications in the biopharma industry. Our SmartEnzymes portfolio consists of enzymes for Antibody Digestion, Antibody Deglycosylation, Antibody Labeling, Glycan Profiling, Affinity Purification and Proteomics.
This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 